One of the more grotesque methods involved in vaccine manufacturing is the collection of fetal bovine serum

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On November 20, 2012, the FDA approved the seasonal influenza vaccine, Flucelvax, manufactured by Novartis

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Viral vaccines prepared in tissue culture or mouse brain have been used in many Asian countries. According to the CDC website, the inactivated mouse brain-derived JE vaccine used in the United States since 1992

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Chickens and their embryos have long been used in the production of vaccines.

These methods were popularized in the 1920s and 1930s by Thomas Rivers and others at the Rockefeller Institute for Medical Research.

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As mentioned above, monkey kidney tissue is used to support the growth of certain viruses used in vaccine production. There remains a huge controversy over using these cells and their role contaminating the polio vaccine in the 1950s.

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